GlycoSens™ 6-Phosphofructokinase (PFK) Assay Kit, Spectrophotometry

The GlycoSens™ 6-Phosphofructokinase (PFK) Assay Kit utilizes a highly stable detection system tailored for accurate profiling of 6-phosphofructokinase. It combines streamlined sample handling with superior signal-to-noise ratios to support dependable biochemical assessment in research applications.

Glycolysis Series

PFK

Assay Buffer; Enzyme Mix; Developer Solution; Standard; Reaction Stop Solution

Tissues, other biological samples

20 μL - 100 μL

Target 6-phosphofructokinase (PFK) in samples undergoes specific enzymatic conversion or chromatic reaction, resulting in a distinct optical absorbance change monitored spectrophotometrically. The variation in absorbance intensity directly correlates with the concentration of target PFK.

Within 2 hours

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

6-Phosphofructokinase

6-Phosphofructokinase; PFK

6-Phosphofructokinase (PFK) is recognized as a fundamental component in biological systems. It functions by transferring phosphate groups from high-energy donor molecules to specific target substrates. Tracking its dynamics facilitates a deeper understanding of cellular responses to external stressors.

Ideal for investigating 6-phosphofructokinase (PFK) activity and metabolic turnover during natural product research, growth factor signaling, and developmental morphogenesis studies.

Rapid Execution; Flexible Sample Inputs; Highly Reproducible Data

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.