GlycoSens™ Phosphofructokinase (PFK) Assay Kit

The GlycoSens™ Phosphofructokinase (PFK) Assay Kit features an optimized micro-volume detection system designed for sensitive measurement of phosphofructokinase (PFK) levels across diverse research samples. The assay components are ready-to-use, ensuring high analytical reproducibility with minimal sample requirements.

Oxidation and Antioxidant Series

PFK

Reaction Buffer; Specific Substrate; Standard; Reaction Stop Solution

Tissue homogenates, cell lysates, biological fluids

50 μL

Quantification of phosphofructokinase (PFK) is established through a specific substrate-mediated reaction. Signal intensity recorded via microplate reader or spectrophotometer is proportionally calibrated against analytical standard curves.

Within 1 hour

340 nm

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Phosphofructokinase

Phosphofructokinase; PFK; Phosphofructo-1-Kinase; 6-Phosphofructokinase

Found widely across biological samples, phosphofructokinase (PFK) is crucial for maintaining systemic homeostasis. This enzyme operates as a critical regulatory switch, modulating the activity of downstream signaling proteins. Therefore, profiling its activity serves as a fundamental strategy for exploring complex biological phenomena.

Specifically engineered for evaluating phosphofructokinase (PFK) dynamics in plant physiology, stress adaptation signaling, and cellular homeostasis research.

High Specificity; Rapid Reaction Protocol; Stringent Quality Control

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.