GlycoSens™ Alanopine Alcohol dehydrogenase (ADH) Assay Kit, UV Method

The GlycoSens™ Alanopine Alcohol dehydrogenase (ADH) Assay Kit utilizes a highly stable detection system tailored for accurate profiling of alanopine alcohol dehydrogenase. It combines streamlined sample handling with superior signal-to-noise ratios to support dependable biochemical assessment in research applications.

Other Series

ADH

Reaction Buffer; Specific Substrate; Standard; Reaction Stop Solution

Tissues, other biological samples

20 μL - 100 μL

Target alanopine alcohol dehydrogenase (ADH) in samples undergoes specific enzymatic conversion or chromatic reaction, resulting in a distinct optical absorbance change monitored spectrophotometrically. The variation in absorbance intensity directly correlates with the concentration of target ADH.

Within 45 minutes

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Alanopine Dehydrogenase

Alanopine Dehydrogenase; ADH; Alanopine Oxidoreductase

As a pivotal biomolecule, alanopine alcohol dehydrogenase (ADH) plays a central role in complex physiological networks. By mediating the transfer of electrons, it strictly regulates intermediate metabolic fluxes. Furthermore, tracking its dynamics facilitates a deeper understanding of cellular responses to external stressors.

Essential for monitoring alanopine alcohol dehydrogenase (ADH) level shifts during molecular biochemistry, metabolic pathway flux analysis, and enzymology studies.

Robust Assay Design; Easy-to-Follow Protocol; Versatile Application Range

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.