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The GlycoSens™ Alcohol Dehydrogenase (ADH) Assay Kit features an optimized micro-volume detection system designed for sensitive measurement of alcohol dehydrogenase (ADH) levels across diverse research samples. The assay components are ready-to-use, ensuring high analytical reproducibility with minimal sample requirements.
Product Type
Fatty Acid Metabolism Series
Target
ADH
Kit Components
Reaction Buffer; Specific Substrate; Standard; Reaction Stop Solution
Sample Types
Tissues, other biological samples
Sample Volume
10 μL - 50 μL
Detection Principle
Quantification of alcohol dehydrogenase (ADH) is established through a specific substrate-mediated reaction. Signal intensity recorded via microplate reader or spectrophotometer is proportionally calibrated against analytical standard curves.
Detection Time
Within 1 hour
Required Equipment & Materials (Self-provided by User)
Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.
Storage
Store at -20°C.
Shipping Conditions
Shipped with ice packs.
Target Information
Full Name
Alcohol Dehydrogenase
Alternate Names
Alcohol Dehydrogenase; ADH; Oxidoreductase
Application Notes
Background
In the context of biological research, alcohol dehydrogenase (ADH) has emerged as a molecule of high interest. Its enzymatic action supports metabolic redox homeostasis and efficient energy conversion pathways. Notably, therefore, profiling its activity serves as a fundamental strategy for exploring complex biological phenomena.
Application
Optimized for high-throughput profiling of alcohol dehydrogenase (ADH) in functional metabolomics, secondary metabolite profiling, and environmental adaptation research.
Key Features & Advantages
Superior Accuracy; Low Background Noise; Highly Stable Signal Output
Operating Instructions
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.
2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.
3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.