GlycoSens™ Alanopine Dehydrogenase (ADH) Assay Kit

The GlycoSens™ Alanopine Dehydrogenase (ADH) Assay Kit utilizes a highly stable detection system tailored for accurate profiling of alanopine dehydrogenase. It combines streamlined sample handling with superior signal-to-noise ratios to support dependable biochemical assessment in research applications.

Glycolysis Series

ADH

Extraction and assay buffer; Substrate Mix A; Substrate Mix B; Reaction developer

Tissues, other biological samples

20 μL - 100 μL

Target alanopine dehydrogenase (ADH) in samples undergoes specific enzymatic conversion or chromatic reaction, resulting in a distinct optical absorbance change monitored spectrophotometrically. The variation in absorbance intensity directly correlates with the concentration of target ADH.

Within 45 minutes

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Alanopine Dehydrogenase

Alanopine Dehydrogenase; ADH; Alanopine Oxidoreductase

Alanopine dehydrogenase (ADH) acts as an essential biochemical entity within diverse cellular pathways. By mediating the transfer of electrons, it strictly regulates intermediate metabolic fluxes. Notably, evaluating its levels provides researchers with invaluable insights into metabolic alterations and health status.

Essential for monitoring alanopine dehydrogenase (ADH) level shifts during molecular biochemistry, metabolic pathway flux analysis, and enzymology studies.

Superior Accuracy; Low Background Noise; Highly Stable Signal Output

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.