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The GlycoSens™ Bound Abscisic Acid (ABA) Assay Kit utilizes a highly stable detection system tailored for accurate profiling of bound abscisic acid. It combines streamlined sample handling with superior signal-to-noise ratios to support dependable biochemical assessment in research applications.
Target bound abscisic acid (ABA) in samples undergoes specific enzymatic conversion or chromatic reaction, resulting in a distinct optical absorbance change monitored spectrophotometrically. The variation in absorbance intensity directly correlates with the concentration of target ABA.
Detection Time
Within 1 hour
Detection Wavelength
450 nm
Required Equipment & Materials (Self-provided by User)
Bound abscisic acid (ABA) is recognized as a fundamental component in biological systems. As a key acidic metabolite, it modulates enzymatic activities and influences intra-cellular signaling. Moreover, evaluating its levels provides researchers with invaluable insights into metabolic alterations and health status.
Application
Designed for comprehensive functional analysis of bound abscisic acid (ABA) within tissue metabolism profiling, coenzyme utilization studies, and biochemical pathway modeling.
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.
2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.
3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.