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The GlycoSens™ Abscisic Acid (ABA) Assay Kit enables precise, direct detection of ABA via enzyme-linked immunosorbent assay (ELISA) measurement. The kit contains ready-to-use reagents optimized for exceptional reproducibility and fast assay turnaround.
The assay uses a competitive ELISA method where target ABA in samples competes with solid-phase antigen for antibody binding. Signal development with substrate yields an absorbance at 450 nm inversely proportional to ABA concentration.
Detection Time
Within 1 hour
Detection Wavelength
450 nm
Required Equipment & Materials (Self-provided by User)
Research highlights abscisic acid (ABA) as a significant participant in key metabolic and regulatory cascades. It functions as a vital organic intermediate, connecting multiple energy-yielding metabolic cycles. Notably, accurate quantification of this target is essential for uncovering underlying pathological and physiological mechanisms.
Application
Ideal for investigating abscisic acid (ABA) activity and metabolic turnover during plant stress adaptation, stomatal control mechanisms, and seed dormancy regulation research.
Key Features & Advantages
Optimized Reaction Dynamics; Broad Sample Compatibility; Stringent Quality Control
Operating Instructions
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.
2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.
3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.