GlycoSens™ Catalase (CAT) Assay Kit, Micro-method

The GlycoSens™ Catalase (CAT) Assay Kit features an optimized micro-volume detection system designed for sensitive measurement of catalase (CAT) levels across diverse research samples. The assay components are ready-to-use, ensuring high analytical reproducibility with minimal sample requirements.

Oxidation and Antioxidant Series

CAT

Assay Buffer; Enzyme Mix; Developer Solution; Standard; Reaction Stop Solution

Serum, plasma, tissue homogenates, cell lysates, biological fluids

10 μL - 50 μL

Quantification of catalase (CAT) is established through a specific substrate-mediated reaction. Signal intensity recorded via microplate reader or spectrophotometer is proportionally calibrated against analytical standard curves.

Within 1 hour

240 nm

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Catalase

Catalase; CAT; Catalase Standard; Catalase Reagent

The presence and activity of catalase (CAT) are closely linked to overall organismal health. This biocatalyst heavily influences cellular redox balance by driving specific oxidation or reduction events. In addition, monitoring these changes is vital for evaluating bioenergetic efficiency and systemic resilience.

Optimized for high-throughput profiling of catalase (CAT) in functional metabolomics, secondary metabolite profiling, and environmental adaptation research.

Optimized Reaction Dynamics; Rapid Reaction Protocol; Minimized Interference

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.