GlycoSens™ Citric Acid (CA) Assay Kit, Spectrophotometry

The GlycoSens™ Citric Acid (CA) Assay Kit provides a robust, high-precision analytical assay platform engineered for quantification of target citric acid in varied biological matrices. Featuring a streamlined protocol, it delivers outstanding linearity and operational speed for routine biochemical assays.

Tricarboxylic Acid Cycle Series

CA

Sample Lysis Buffer; Substrate; Detection Reagent; Standard

Tissues, other biological samples

10 μL - 50 μL

The assay relies on the distinct spectroscopic optical characteristics of citric acid. Upon reaction with specialized chromogenic reagents, the resulting optical density shifts measured at target wavelengths directly reflect CA content in the sample.

Within 45 minutes

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Citric Acid

Citrate; CA; Citric Acid

As a pivotal biomolecule, citric acid (CA) plays a central role in complex physiological networks. Its transport and utilization are critical for comprehensive physiological profiling and energy balance. Notably, investigating its functional state is crucial for identifying novel biomarkers and therapeutic targets.

Optimized for high-throughput profiling of citric acid (CA) in functional metabolomics, secondary metabolite profiling, and environmental adaptation research.

High Specificity; Micro-Volume Optimization; Broad Sample Compatibility

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.