GlycoSens™ Citric Acid (CA) Assay Kit, Micro-method

The GlycoSens™ Citric Acid (CA) Assay Kit utilizes a highly stable detection system tailored for accurate profiling of citric acid. It combines streamlined sample handling with superior signal-to-noise ratios to support dependable biochemical assessment in research applications.

Tricarboxylic Acid Cycle Series

CA

Extraction and assay buffer; Substrate Mix A; Substrate Mix B; Reaction developer

Tissues, other biological samples

10 μL - 50 μL

Target citric acid (CA) in samples undergoes specific enzymatic conversion or chromatic reaction, resulting in a distinct optical absorbance change monitored spectrophotometrically. The variation in absorbance intensity directly correlates with the concentration of target CA.

Within 45 minutes

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Citrate

Citrate; CA

The presence and activity of citric acid (CA) are closely linked to overall organismal health. Its transport and utilization are critical for comprehensive physiological profiling and energy balance. In addition, therefore, profiling its activity serves as a fundamental strategy for exploring complex biological phenomena.

Designed for comprehensive functional analysis of citric acid (CA) within tissue metabolism profiling, coenzyme utilization studies, and biochemical pathway modeling.

Rapid Execution; Flexible Sample Inputs; Highly Reproducible Data

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.