GlycoSens™ Citric Acid (CA) Assay Kit, Sulfosalicylic Acid Method

The GlycoSens™ Citric Acid (CA) Assay Kit features an optimized micro-volume detection system designed for sensitive measurement of citric acid (CA) levels across diverse research samples. The assay components are ready-to-use, ensuring high analytical reproducibility with minimal sample requirements.

Tricarboxylic Acid Cycle Series

CA

Sample Lysis Buffer; Substrate; Detection Reagent; Standard

Tissues, other biological samples

10 μL - 50 μL

Quantification of citric acid (CA) is established through a specific substrate-mediated reaction. Signal intensity recorded via microplate reader or spectrophotometer is proportionally calibrated against analytical standard curves.

30-60 minutes

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Citric Acid

Citrate; CA; Citric Acid

Citric acid (CA) is an integral element required for robust cellular function and metabolism. As a key acidic metabolite, it modulates enzymatic activities and influences intra-cellular signaling. Moreover, consequently, robust measurement of this parameter is highly beneficial for comprehensive functional profiling.

Specifically engineered for evaluating citric acid (CA) dynamics in plant physiology, stress adaptation signaling, and cellular homeostasis research.

Robust Assay Design; Easy-to-Follow Protocol; Versatile Application Range

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.