GlycoSens™ Carbonic Anhydrase (CA) Assay Kit, Micro-method

The GlycoSens™ Carbonic Anhydrase (CA) Assay Kit provides a robust, high-precision analytical assay platform engineered for quantification of target carbonic anhydrase in varied biological matrices. Featuring a streamlined protocol, it delivers outstanding linearity and operational speed for routine biochemical assays.

Other Series

CA

Reaction Buffer; Specific Substrate; Standard; Reaction Stop Solution

Tissues, other biological samples

5 μL - 20 μL

The assay relies on the distinct spectroscopic optical characteristics of carbonic anhydrase. Upon reaction with specialized chromogenic reagents, the resulting optical density shifts measured at target wavelengths directly reflect CA content in the sample.

Within 2 hours

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Carbonic Anhydrase

Carbonic Anhydrase; CA

Carbonic anhydrase (CA) is an integral element required for robust cellular function and metabolism. This catalytic protein strictly regulates substrate turnover to meet the dynamic physiological needs of the cell. As a result, consequently, robust measurement of this parameter is highly beneficial for comprehensive functional profiling.

Essential for monitoring carbonic anhydrase (CA) level shifts during molecular biochemistry, metabolic pathway flux analysis, and enzymology studies.

Optimized Reaction Dynamics; Excellent Reproducibility; Ready-to-Use Reagents

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.