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GlycoSens™ Complement C4 Assay Kit

CAT#: GLJF-0926-HX1496

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Specifications
Product Description
The GlycoSens™ Complement C4 Assay Kit utilizes a highly stable detection system tailored for accurate profiling of complement C4. It combines streamlined sample handling with superior signal-to-noise ratios to support dependable biochemical assessment in research applications.
Product Type
Other Series
Target
Complement C4
Kit Components
Extraction and assay buffer; Substrate Mix A; Substrate Mix B; Reaction developer
Sample Types
Tissues, other biological samples
Sample Volume
20 μL - 100 μL
Detection Principle
Target complement C4 in samples undergoes specific enzymatic conversion or chromatic reaction, resulting in a distinct optical absorbance change monitored spectrophotometrically. The variation in absorbance intensity directly correlates with the concentration of target complement C4.
Detection Time
30-60 minutes
Required Equipment & Materials (Self-provided by User)
Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.
Storage
Store at -20°C.
Shipping Conditions
Shipped with ice packs.
Target Information
Full Name
Complement C4
Alternate Names
Complement C4
Application Notes
Background
Complement C4 is an integral element required for robust cellular function and metabolism. Its concentration levels are highly indicative of the functional state and metabolic vigor of the sample. In addition, consequently, robust measurement of this parameter is highly beneficial for comprehensive functional profiling.
Application
Essential for monitoring complement c4 level shifts during molecular biochemistry, metabolic pathway flux analysis, and enzymology studies.
Key Features & Advantages
Optimized Reaction Dynamics; Excellent Reproducibility; Ready-to-Use Reagents
Operating Instructions
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.
For Research Use Only.
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