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The GlycoSens™ Complement C4 Assay Kit provides a robust, high-precision analytical assay platform engineered for quantification of target complement C4 in varied biological matrices. Featuring a streamlined protocol, it delivers outstanding linearity and operational speed for routine biochemical assays.
The assay relies on the distinct spectroscopic optical characteristics of complement C4. Upon reaction with specialized chromogenic reagents, the resulting optical density shifts measured at target wavelengths directly reflect complement C4 content in the sample.
Detection Time
Within 2 hours
Required Equipment & Materials (Self-provided by User)
Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.
Storage
Store at -20°C.
Shipping Conditions
Shipped with ice packs.
Target Information
Full Name
Complement C4
Alternate Names
Complement C4
Application Notes
Background
As a pivotal biomolecule, complement C4 plays a central role in complex physiological networks. It actively circulates within the biological matrix, acting as a crucial building block or signaling molecule. Notably, assessing this component offers a reliable parameter for agricultural research or biological studies.
Application
Ideal for investigating complement c4 activity and metabolic turnover during natural product research, growth factor signaling, and developmental morphogenesis studies.
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.
2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.
3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.