GlycoSens™ Glucoamylase Assay Kit, Micro-method

The GlycoSens™ Glucoamylase Assay Kit utilizes a highly stable detection system tailored for accurate profiling of glucoamylase. It combines streamlined sample handling with superior signal-to-noise ratios to support dependable biochemical assessment in research applications.

Carbohydrate Metabolism Series

Glucoamylase

Extraction Buffer; Reaction Reagent A; Reaction Reagent B; Standard

Tissues, other biological samples

10 μL - 50 μL

Target glucoamylase in samples undergoes specific enzymatic conversion or chromatic reaction, resulting in a distinct optical absorbance change monitored spectrophotometrically. The variation in absorbance intensity directly correlates with the concentration of target glucoamylase.

Within 45 minutes

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Glucoamylase

Glucoamylase

Research highlights glucoamylase as a significant participant in key metabolic and regulatory cascades. It governs molecular turnover by catalyzing the cleavage of specific chemical bonds within its target substrates. Notably, assessing this component offers a reliable parameter for agricultural research or biological studies.

Optimized for high-throughput profiling of glucoamylase in functional metabolomics, secondary metabolite profiling, and environmental adaptation research.

High Specificity; Micro-Volume Optimization; Broad Sample Compatibility

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.