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The GlycoSens™ γ-Glutamate Kinase (γ-GK) Assay Kit features an optimized micro-volume detection system designed for sensitive measurement of γ-glutamate kinase (γ-GK) levels across diverse research samples. The assay components are ready-to-use, ensuring high analytical reproducibility with minimal sample requirements.
Product Type
Other Series
Target
γ-GK
Kit Components
Reaction Buffer; Specific Substrate; Standard; Reaction Stop Solution
Sample Types
Tissues, other biological samples
Sample Volume
5 μL - 20 μL
Detection Principle
Quantification of γ-glutamate kinase (γ-GK) is established through a specific substrate-mediated reaction. Signal intensity recorded via microplate reader or spectrophotometer is proportionally calibrated against analytical standard curves.
Detection Time
Within 45 minutes
Required Equipment & Materials (Self-provided by User)
Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.
As a pivotal biomolecule, γ-glutamate kinase (γ-GK) plays a central role in complex physiological networks. It functions by transferring phosphate groups from high-energy donor molecules to specific target substrates. In addition, tracking its dynamics facilitates a deeper understanding of cellular responses to external stressors.
Application
Specifically engineered for evaluating γ-glutamate kinase (γ-GK) dynamics in plant physiology, stress adaptation signaling, and cellular homeostasis research.
Key Features & Advantages
Robust Assay Design; Easy-to-Follow Protocol; Versatile Application Range
Operating Instructions
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.
2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.
3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.