GlycoSens™ Glutamate-oxaloacetate Transaminase/Aspartate Aminotransferase (GOT/AST) Assay Kit

The GlycoSens™ Glutamate-oxaloacetate Transaminase/Aspartate Aminotransferase (GOT/AST) Assay Kit offers an advanced quantitative methodology for evaluating glutamate-oxaloacetate transaminase/aspartate aminotransferase concentration with high specificity. Designed to minimize matrix background interference, this kit facilitates high-throughput scientific profiling and consistent experimental turnaround.

Nitrogen Metabolism Series

GOT/AST

Sample Lysis Buffer; Substrate; Detection Reagent; Standard

Tissues, other biological samples

20 μL - 100 μL

The system measures chromatic product formation derived from target glutamate-oxaloacetate transaminase/aspartate aminotransferase activity. Absorbance changes tracked at optimal wavelengths allow precise quantitative determination of GOT/AST levels.

Within 1 hour

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Glutamate-oxaloacetate Transaminase/Aspartate Aminotransferase

Glutamate-oxaloacetate Transaminase/Aspartate Aminotransferase; GOT/AST

Glutamate-oxaloacetate transaminase/aspartate aminotransferase (GOT/AST) is an integral element required for robust cellular function and metabolism. By lowering activation energy barriers, it drives essential chemical reactions that sustain cellular life. Consequently, therefore, profiling its activity serves as a fundamental strategy for exploring complex biological phenomena.

Ideal for investigating glutamate-oxaloacetate transaminase/aspartate aminotransferase (GOT/AST) activity and metabolic turnover during natural product research, growth factor signaling, and developmental morphogenesis studies.

High Specificity; Stable Reaction; Broad Sample Compatibility

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.