GlycoSens™ Glutamate-oxaloacetate Transaminase/Aspartate Aminotransferase (GOT/AST) Assay Kit, Micro-method

The GlycoSens™ Glutamate-oxaloacetate Transaminase/Aspartate Aminotransferase (GOT/AST) Assay Kit features an optimized micro-volume detection system designed for sensitive measurement of glutamate-oxaloacetate transaminase/aspartate aminotransferase (GOT/AST) levels across diverse research samples. The assay components are ready-to-use, ensuring high analytical reproducibility with minimal sample requirements.

Amino Acid Metabolism Series

GOT/AST

Extraction and assay buffer; Substrate Mix A; Substrate Mix B; Reaction developer

Tissues, other biological samples

10 μL - 50 μL

Quantification of glutamate-oxaloacetate transaminase/aspartate aminotransferase (GOT/AST) is established through a specific substrate-mediated reaction. Signal intensity recorded via microplate reader or spectrophotometer is proportionally calibrated against analytical standard curves.

Within 2 hours

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Glutamate-oxaloacetate Transaminase/Aspartate Aminotransferase

Glutamate-oxaloacetate Transaminase/Aspartate Aminotransferase; GOT/AST

Glutamate-oxaloacetate transaminase/aspartate aminotransferase (GOT/AST) acts as an essential biochemical entity within diverse cellular pathways. It selectively processes distinct biochemical substrates, maintaining the efficiency of cellular networks. In addition, therefore, profiling its activity serves as a fundamental strategy for exploring complex biological phenomena.

Tailored for accurate quantification of glutamate-oxaloacetate transaminase/aspartate aminotransferase (GOT/AST) across cell biology, organelle bioenergetics, and intracellular signaling cascades.

Superior Accuracy; Low Background Noise; Highly Stable Signal Output

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.