GlycoSens™ Glutamate-oxaloacetate Transaminase/Aspartate Aminotransferase (GOT/AST) Assay Kit, Spectrophotometry

The GlycoSens™ Glutamate-oxaloacetate Transaminase/Aspartate Aminotransferase (GOT/AST) Assay Kit utilizes a highly stable detection system tailored for accurate profiling of glutamate-oxaloacetate transaminase/aspartate aminotransferase. It combines streamlined sample handling with superior signal-to-noise ratios to support dependable biochemical assessment in research applications.

Amino Acid Metabolism Series

GOT/AST

Sample Lysis Buffer; Substrate; Detection Reagent; Standard

Tissues, other biological samples

20 μL - 100 μL

Target glutamate-oxaloacetate transaminase/aspartate aminotransferase (GOT/AST) in samples undergoes specific enzymatic conversion or chromatic reaction, resulting in a distinct optical absorbance change monitored spectrophotometrically. The variation in absorbance intensity directly correlates with the concentration of target GOT/AST.

Within 2 hours

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Glutamate-oxaloacetate Transaminase/Aspartate Aminotransferase

Glutamate-oxaloacetate Transaminase/Aspartate Aminotransferase; GOT/AST

Glutamate-oxaloacetate transaminase/aspartate aminotransferase (GOT/AST) is an integral element required for robust cellular function and metabolism. By lowering activation energy barriers, it drives essential chemical reactions that sustain cellular life. As a result, tracking its dynamics facilitates a deeper understanding of cellular responses to external stressors.

Optimized for high-throughput profiling of glutamate-oxaloacetate transaminase/aspartate aminotransferase (GOT/AST) in functional metabolomics, secondary metabolite profiling, and environmental adaptation research.

Rapid Reaction Protocol; Stringent Quality Control; Enhanced Sensitivity

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.