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The GlycoSens™ Glutathione S-Transferase (GST) Assay Kit offers an advanced quantitative methodology for evaluating glutathione S-transferase concentration with high specificity. Designed to minimize matrix background interference, this kit facilitates high-throughput scientific profiling and consistent experimental turnaround.
The system measures chromatic product formation derived from target glutathione s-transferase activity. Absorbance changes tracked at optimal wavelengths allow precise quantitative determination of GST levels.
Detection Time
Within 45 minutes
Required Equipment & Materials (Self-provided by User)
Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.
Storage
Store at -20°C.
Shipping Conditions
Shipped with ice packs.
Target Information
Full Name
Glutathione S-Transferase
Alternate Names
Glutathione S-Transferase; GST
Application Notes
Background
Glutathione S-transferase (GST) acts as an essential biochemical entity within diverse cellular pathways. It significantly contributes to the cellular antioxidant network, enhancing tolerance against oxidative injury. Furthermore, monitoring these changes is vital for evaluating bioenergetic efficiency and systemic resilience.
Application
Widely applicable for tracking glutathione S-transferase (GST) fluctuations across agricultural biotechnology, crop growth regulation, and physiological stress responses.
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.
2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.
3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.