GlycoSens™ Hydroxyl Radical Scavenging Capacity Assay Kit, Spectrophotometry

The GlycoSens™ Hydroxyl Radical Scavenging Capacity Assay Kit provides a robust, high-precision analytical assay platform engineered for quantification of target hydroxyl radical scavenging capacity in varied biological matrices. Featuring a streamlined protocol, it delivers outstanding linearity and operational speed for routine biochemical assays.

Oxidation and Antioxidant Series

Assay Buffer; Enzyme Mix; Developer Solution; Standard; Reaction Stop Solution

Tissue homogenates, cellular lysates, microbial extracts

10 μL - 50 μL

The assay relies on the distinct spectroscopic optical characteristics of hydroxyl radical scavenging capacity. Upon reaction with specialized chromogenic reagents, the resulting optical density shifts measured at target wavelengths directly reflect hydroxyl radical scavenging capacity in the sample.

Within 2 hours

536 nm

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Functioning at the core of various biological processes, hydroxyl radical scavenging capacity is a key subject of biochemical analysis. It actively circulates within the biological matrix, acting as a crucial building block or signaling molecule. Moreover, consequently, robust measurement of this parameter is highly beneficial for comprehensive functional profiling.

Tailored for accurate quantification of hydroxyl radical scavenging capacity across cell biology, organelle bioenergetics, and intracellular signaling cascades.

Rapid Execution; Flexible Sample Inputs; Highly Reproducible Data

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.