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The GlycoSens™ Isopentenyladenosine (2iPR) Assay Kit utilizes a highly stable detection system tailored for accurate profiling of isopentenyladenosine. It combines streamlined sample handling with superior signal-to-noise ratios to support dependable biochemical assessment in research applications.
Target isopentenyladenosine (2iPR) in samples undergoes specific enzymatic conversion or chromatic reaction, resulting in a distinct optical absorbance change monitored spectrophotometrically. The variation in absorbance intensity directly correlates with the concentration of target 2iPR.
Detection Time
Within 1 hour
Detection Wavelength
254 nm
Required Equipment & Materials (Self-provided by User)
The presence and activity of isopentenyladenosine (2iPR) are closely linked to overall organismal health. It forms a crucial part of the cellular inventory, dynamically responding to internal and external stimuli. In addition, tracking its dynamics facilitates a deeper understanding of cellular responses to external stressors.
Application
Specifically engineered for evaluating isopentenyladenosine (2iPR) dynamics in plant physiology, stress adaptation signaling, and cellular homeostasis research.
Key Features & Advantages
High Specificity; Excellent Reproducibility; Stringent Quality Control
Operating Instructions
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.
2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.
3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.