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GlycoSens™ Isopentenyladenosine (2iPR) Assay Kit

CAT#: GLJF-0926-HX28

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Specifications
Product Description
The GlycoSens™ Isopentenyladenosine (2iPR) Assay Kit utilizes a highly stable detection system tailored for accurate profiling of isopentenyladenosine. It combines streamlined sample handling with superior signal-to-noise ratios to support dependable biochemical assessment in research applications.
Product Type
Plant Hormone Series
Target
2iPR
Kit Components
Substrate Mix A; Substrate Mix B; Standard; Reaction Stop Solution
Sample Types
Tissue homogenates, cell lysates, biological fluids
Sample Volume
10 μL - 50 μL
Detection Principle
Target isopentenyladenosine (2iPR) in samples undergoes specific enzymatic conversion or chromatic reaction, resulting in a distinct optical absorbance change monitored spectrophotometrically. The variation in absorbance intensity directly correlates with the concentration of target 2iPR.
Detection Time
Within 1 hour
Detection Wavelength
254 nm
Required Equipment & Materials (Self-provided by User)
High-performance liquid chromatograph, centrifuge, chromatographic column, adjustable micropipettes, double-distilled water.
Storage
Store at -20°C.
Shipping Conditions
Shipped with ice packs.
Target Information
Full Name
Isopentenyladenosine
Alternate Names
Isopentenyladenosine; 2iPR; Isopentenyladenosine Standard; Isopentenyladenosine Reagent
Application Notes
Background
The presence and activity of isopentenyladenosine (2iPR) are closely linked to overall organismal health. It forms a crucial part of the cellular inventory, dynamically responding to internal and external stimuli. In addition, tracking its dynamics facilitates a deeper understanding of cellular responses to external stressors.
Application
Specifically engineered for evaluating isopentenyladenosine (2iPR) dynamics in plant physiology, stress adaptation signaling, and cellular homeostasis research.
Key Features & Advantages
High Specificity; Excellent Reproducibility; Stringent Quality Control
Operating Instructions
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.
For Research Use Only.
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