GlycoSens™ Mitochondrial Malate Dehydrogenase (MDHm) Assay Kit, Micro-method

The GlycoSens™ Mitochondrial Malate Dehydrogenase(MDHm) Assay Kit offers an advanced quantitative methodology for evaluating mitochondrial malate dehydrogenase concentration with high specificity. Designed to minimize matrix background interference, this kit facilitates high-throughput scientific profiling and consistent experimental turnaround.

Tricarboxylic Acid Cycle Series

MDHm

Sample Lysis Buffer; Substrate; Detection Reagent; Standard

Tissues, other biological samples

20 μL - 100 μL

The system measures chromatic product formation derived from target mitochondrial malate dehydrogenase activity. Absorbance changes tracked at optimal wavelengths allow precise quantitative determination of MDHm levels.

30-60 minutes

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Mitochondrial Malate Dehydrogenase

Mitochondrial Malate Dehydrogenase; MDHm; Mitochondrial Malate Oxidoreductase

The presence and activity of mitochondrial malate dehydrogenase (MDHm) are closely linked to overall organismal health. By mediating the transfer of electrons, it strictly regulates intermediate metabolic fluxes. Notably, detailed analysis of this target empowers researchers to monitor disease progression or developmental stages effectively.

Widely applicable for tracking mitochondrial malate dehydrogenase (MDHm) fluctuations across agricultural biotechnology, crop growth regulation, and physiological stress responses.

High Sensitivity & Specificity; Streamlined Workflow; Consistent Batch-to-Batch Performance

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.