GlycoSens™ Mitochondrial Malate Dehydrogenase (MDHm) Assay Kit, Spectrophotometry

The GlycoSens™ Mitochondrial Malate Dehydrogenase(MDHm) Assay Kit features an optimized micro-volume detection system designed for sensitive measurement of mitochondrial malate dehydrogenase (MDHm) levels across diverse research samples. The assay components are ready-to-use, ensuring high analytical reproducibility with minimal sample requirements.

Tricarboxylic Acid Cycle Series

MDHm

Reaction Buffer; Specific Substrate; Standard; Reaction Stop Solution

Tissues, other biological samples

20 μL - 100 μL

Quantification of mitochondrial malate dehydrogenase (MDHm) is established through a specific substrate-mediated reaction. Signal intensity recorded via microplate reader or spectrophotometer is proportionally calibrated against analytical standard curves.

Within 1 hour

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Mitochondrial Malate Dehydrogenase

Mitochondrial Malate Dehydrogenase; MDHm; Mitochondrial Malate Oxidoreductase

Mitochondrial malate dehydrogenase (MDHm) acts as an essential biochemical entity within diverse cellular pathways. By mediating the transfer of electrons, it strictly regulates intermediate metabolic fluxes. Tracking its dynamics facilitates a deeper understanding of cellular responses to external stressors.

Ideal for investigating mitochondrial malate dehydrogenase (MDHm) activity and metabolic turnover during natural product research, growth factor signaling, and developmental morphogenesis studies.

Optimized Reaction Dynamics; Excellent Reproducibility; Ready-to-Use Reagents

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.