GlycoSens™ Mitochondrial Respiratory Chain Complex IV Assay Kit, Micro-method

The GlycoSens™ Mitochondrial Respiratory Chain Complex IV Assay Kit enables precise, direct detection of mitochondrial respiratory chain complex IV via micro-volume spectrophotometric measurement. The kit contains ready-to-use reagents optimized for exceptional reproducibility and fast assay turnaround.

Oxidative Phosphorylation Series

Mitochondrial Respiratory Chain Complex IV

Mitochondrial Isolation Buffer; Lysis Reagent; Protease Inhibitor Cocktail; Wash Buffer

Isolated mitochondria, cell lysates, tissue homogenates

10 μL - 50 μL

Complex IV catalyzes the oxidation of reduced cytochrome C into oxidized cytochrome C. Reduced cytochrome c absorbs strongly at 550 nm; hence, the decrease rate of absorbance at 550 nm measures Complex IV enzyme activity.

Within 1 hour

550 nm

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Mitochondrial Respiratory Chain Complex IV

Complex IV; Mitochondrial Respiratory Chain Complex IV Standard; Mitochondrial Respiratory Chain Complex IV Reagent; Cytochrome C Oxidase

Found widely across biological samples, mitochondrial respiratory chain complex IV is crucial for maintaining systemic homeostasis. Its structural integrity and enzymatic turnover are critical determinants of mitochondrial health and reactive oxygen species generation. In addition, monitoring these changes is vital for evaluating bioenergetic efficiency and systemic resilience.

Widely applicable for tracking mitochondrial respiratory chain complex IV fluctuations across cellular respiration studies, electron transport dynamics, and metabolic flux profiling.

High Specificity; Excellent Reproducibility; Minimized Interference

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.