GlycoSens™ Mitochondrial Respiratory Chain Complex IV Assay Kit, Spectrophotometry

The GlycoSens™ Mitochondrial Respiratory Chain Complex IV Assay Kit offers a highly reliable and sensitive spectrophotometric platform for quantifying mitochondrial respiratory chain complex IV across varied biological matrices. Designed for rapid operation and high precision, this assay ensures reproducible quantification with low sample volume requirements.

Oxidative Phosphorylation Series

Mitochondrial Respiratory Chain Complex IV

Mitochondrial Isolation Buffer; Lysis Reagent; Protease Inhibitor Cocktail; Wash Buffer

Isolated mitochondria, cell lysates, tissue homogenates

10 μL - 50 μL

Complex IV catalyzes the oxidation of reduced cytochrome C into oxidized cytochrome C. Reduced cytochrome c absorbs strongly at 550 nm. Hence, the decrease rate of absorbance at 550 nm measures Complex IV enzyme activity.

Within 1 hour

550 nm

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Mitochondrial Respiratory Chain Complex IV

Complex IV; Mitochondrial Respiratory Chain Complex IV Standard; Cytochrome C Oxidase; Cyt c Oxidase

The presence and activity of mitochondrial respiratory chain complex IV are closely linked to overall organismal health. It plays a critical role in cellular bioenergetics by maintaining the proton motive force across the inner mitochondrial membrane. Consequently, monitoring these changes is vital for evaluating bioenergetic efficiency and systemic resilience.

Essential for monitoring mitochondrial respiratory chain complex IV activity shifts during cellular respiration, terminal electron transfer signaling, and organelle bioenergetics research.

High Specificity; Excellent Reproducibility; Stringent Quality Control

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.