GlycoSens™ Mixed-Function Oxidase (MFO) Assay Kit

The GlycoSens™ Mixed-Function Oxidase (MFO) Assay Kit utilizes a highly stable detection system tailored for accurate profiling of mixed-function oxidase. It combines streamlined sample handling with superior signal-to-noise ratios to support dependable biochemical assessment in research applications.

Oxidation and Antioxidant Series

MFO

Substrate Mix A; Substrate Mix B; Standard; Reaction Stop Solution

Tissue homogenates, cell lysates, biological fluids

10 μL - 50 μL

Target mixed-function oxidase (MFO) in samples undergoes specific enzymatic conversion or chromatic reaction, resulting in a distinct optical absorbance change monitored spectrophotometrically. The variation in absorbance intensity directly correlates with the concentration of target MFO.

Within 1 hour

400 nm

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Mixed-Function Oxidase

Mixed-Function Oxidase; MFO; Mixed-Function Oxidase Standard; Mixed-Function Oxidase Reagent

The presence and activity of mixed-function oxidase (MFO) are closely linked to overall organismal health. Through its catalytic activity, it ensures the safe processing of oxidative metabolites within the cell. Tracking its dynamics facilitates a deeper understanding of cellular responses to external stressors.

Tailored for accurate quantification of mixed-function oxidase (MFO) across cell biology, organelle bioenergetics, and intracellular signaling cascades.

Optimized Reaction Dynamics; Excellent Reproducibility; Stringent Quality Control

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.