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The GlycoSens™ Soil Fungi Total Content Assay Kit utilizes a highly stable detection system tailored for accurate profiling of soil fungi total content. It combines streamlined sample handling with superior signal-to-noise ratios to support dependable biochemical assessment in research applications.
Product Type
Soil Series
Kit Components
Extraction Solvent; Hydrolysis Solution; Chromogenic Reagent; Standard
Sample Types
Soil, sediments, solid substrates
Sample Volume
10 μL - 50 μL
Detection Principle
Target soil fungi total content in samples undergoes specific enzymatic conversion or chromatic reaction, resulting in a distinct optical absorbance change monitored spectrophotometrically. The variation in absorbance intensity directly correlates with the concentration of target soil fungi total content.
Detection Time
Within 1 hour
Required Equipment & Materials (Self-provided by User)
Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.
Storage
Store at -20°C.
Shipping Conditions
Shipped with ice packs.
Application Notes
Background
Functioning at the core of various biological processes, soil fungi total content is a key subject of biochemical analysis. This parameter is vital for evaluating environmental changes and soil nutrient turnover. In addition, consequently, robust measurement of this parameter is highly beneficial for comprehensive functional profiling.
Application
Essential for monitoring soil fungi total content level shifts during molecular biochemistry, metabolic pathway flux analysis, and enzymology studies.
Key Features & Advantages
Robust Assay Design; Rigorously Validated Performance; Versatile Application Range
Operating Instructions
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.
2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.
3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.