GlycoSens™ Pyruvate Decarboxylase (PDC) Assay Kit, Spectrophotometry

The GlycoSens™ Pyruvate Decarboxylase (PDC) Assay Kit offers an advanced quantitative methodology for evaluating pyruvate decarboxylase concentration with high specificity. Designed to minimize matrix background interference, this kit facilitates high-throughput scientific profiling and consistent experimental turnaround.

Fatty Acid Metabolism Series

PDC

Assay Buffer; Enzyme Mix; Developer Solution; Standard; Reaction Stop Solution

Tissues, other biological samples

10 μL - 50 μL

The system measures chromatic product formation derived from target pyruvate decarboxylase activity. Absorbance changes tracked at optimal wavelengths allow precise quantitative determination of PDC levels.

30-60 minutes

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Pyruvate Decarboxylase

Pyruvate Decarboxylase; PDC

The presence and activity of pyruvate decarboxylase (PDC) are closely linked to overall organismal health. It selectively processes distinct biochemical substrates, maintaining the efficiency of cellular networks. Tracking its dynamics facilitates a deeper understanding of cellular responses to external stressors.

Ideal for investigating pyruvate decarboxylase (PDC) activity and metabolic turnover during natural product research, growth factor signaling, and developmental morphogenesis studies.

Robust Assay Design; Easy-to-Follow Protocol; High Signal-to-Noise Ratio

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.