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The GlycoSens™ Soil α-Glucosidase (S-α-GC) Assay Kit provides a robust, high-precision analytical assay platform engineered for quantification of target soil α-glucosidase in varied biological matrices. Featuring a streamlined protocol, it delivers outstanding linearity and operational speed for routine biochemical assays.
Product Type
Soil Series
Target
S-α-GC
Kit Components
Extraction Buffer; Reaction Reagent A; Reaction Reagent B; Standard
Sample Types
Soil, sediments, solid substrates
Sample Volume
10 μL - 50 μL
Detection Principle
The assay relies on the distinct spectroscopic optical characteristics of soil α-glucosidase. Upon reaction with specialized chromogenic reagents, the resulting optical density shifts measured at target wavelengths directly reflect S-α-GC content in the sample.
Detection Time
Within 45 minutes
Required Equipment & Materials (Self-provided by User)
Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.
Storage
Store at -20°C.
Shipping Conditions
Shipped with ice packs.
Target Information
Full Name
Soil α-Glucosidase
Alternate Names
Soil α-Glucosidase; S-α-GC
Application Notes
Background
Soil α-glucosidase (S-α-GC) is an integral element required for robust cellular function and metabolism. Its dynamics closely reflect soil fertility, organic matter decomposition, and ecosystem health. Consequently, detailed analysis of this target empowers researchers to monitor disease progression or developmental stages effectively.
Application
Essential for monitoring soil α-glucosidase (S-α-GC) level shifts during molecular biochemistry, metabolic pathway flux analysis, and enzymology studies.
Key Features & Advantages
Superior Accuracy; Low Background Noise; Highly Stable Signal Output
Operating Instructions
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.
2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.
3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.