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The GlycoSens™ Soil α-Glucosidase (S-α-GC) Assay Kit provides a robust, high-precision analytical assay platform engineered for quantification of target soil α-glucosidase in varied biological matrices. Featuring a streamlined protocol, it delivers outstanding linearity and operational speed for routine biochemical assays.
Product Type
Soil Series
Target
S-α-GC
Kit Components
Reaction Buffer; Specific Substrate; Standard; Reaction Stop Solution
Sample Types
Soil, sediments, solid substrates
Sample Volume
10 μL - 50 μL
Detection Principle
The assay relies on the distinct spectroscopic optical characteristics of soil α-glucosidase. Upon reaction with specialized chromogenic reagents, the resulting optical density shifts measured at target wavelengths directly reflect S-α-GC content in the sample.
Detection Time
30-60 minutes
Required Equipment & Materials (Self-provided by User)
Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.
Storage
Store at -20°C.
Shipping Conditions
Shipped with ice packs.
Target Information
Full Name
Soil α-Glucosidase
Alternate Names
Soil α-Glucosidase; S-α-GC
Application Notes
Background
Functioning at the core of various biological processes, soil α-glucosidase (S-α-GC) is a key subject of biochemical analysis. This parameter is vital for evaluating environmental changes and soil nutrient turnover. Moreover, accurate quantification of this target is essential for uncovering underlying pathological and physiological mechanisms.
Application
Designed for comprehensive functional analysis of soil α-glucosidase (S-α-GC) within tissue metabolism profiling, coenzyme utilization studies, and biochemical pathway modeling.
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.
2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.
3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.