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The GlycoSens™ Total Protein (TP) Assay Kit features an optimized micro-volume detection system designed for sensitive measurement of total protein (TP) levels across diverse research samples. The assay components are ready-to-use, ensuring high analytical reproducibility with minimal sample requirements.
Quantification of total protein (TP) is established through a specific substrate-mediated reaction. Signal intensity recorded via microplate reader or spectrophotometer is proportionally calibrated against analytical standard curves.
Detection Time
Within 1 hour
Detection Wavelength
562 nm
Required Equipment & Materials (Self-provided by User)
Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.
Storage
Store at -20°C.
Shipping Conditions
Shipped with ice packs.
Target Information
Full Name
Total Protein
Alternate Names
Total Protein; TP; Total Protein Standard; Total Protein Reagent
Application Notes
Background
Found widely across biological samples, total protein (TP) is crucial for maintaining systemic homeostasis. By aggregating related molecular species, it offers a robust index for general biological vitality. Moreover, investigating its functional state is crucial for identifying novel biomarkers and therapeutic targets.
Application
Specifically engineered for evaluating total protein (TP) dynamics in plant physiology, stress adaptation signaling, and cellular homeostasis research.
Key Features & Advantages
Stable Signal Output; Rapid Reaction Protocol; Ready-to-Use Reagents
Operating Instructions
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.
2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.
3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.