GlycoSens™ Transhydrogenase-2 (TH-2) Assay Kit

The GlycoSens™ Transhydrogenase-2 (TH-2) Assay Kit offers a highly reliable and sensitive micro-volume spectrophotometric platform for quantifying transhydrogenase-2 (TH-2) across varied biological matrices. Designed for rapid operation and high precision, this assay ensures reproducible quantification with low sample volume requirements.

Oxidative Phosphorylation Series

TH-2

Extraction and assay buffer; Substrate Mix A; Substrate Mix B; Reaction developer / stabilizer

Tissue homogenates, cellular lysates, microbial extracts

10 μL - 50 μL

Transhydrogenase-2 (TH-2) catalyzes hydride transfer from NADPH to NAD⁺. Synthetic substrate analog 3-acetylpyridine adenine dinucleotide (APAD⁺) is reduced to APADH, which displays a unique optical absorbance peak at 375 nm. Activity is quantified by tracking OD increase at 375 nm.

Within 1 hour

375 nm

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Transhydrogenase-2

TH-2; Mitochondrial Complex VI / Reverse Transhydrogenase; Transhydrogenase-2

Transhydrogenase-2 (TH-2) is an integral element required for robust cellular function and metabolism. By serving as a metabolic node, it integrates diverse biochemical inputs into unified physiological responses. Accurate quantification of this target is essential for uncovering underlying pathological and physiological mechanisms.

Specifically engineered for evaluating transhydrogenase-2 (TH-2) dynamics in cell biology, organelle bioenergetics, and mitochondrial respiratory chain research.

High Specificity; Micro-Volume Optimization; Broad Sample Compatibility

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.