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GlycoSens™ Vegetable Nitrate Assay Kit

CAT#: GLJF-0926-HX1422

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Specifications
Product Description
The GlycoSens™ Vegetable Nitrate Assay Kit features an optimized micro-volume detection system designed for sensitive measurement of vegetable nitrate levels across diverse research samples. The assay components are ready-to-use, ensuring high analytical reproducibility with minimal sample requirements.
Product Type
Other Series
Kit Components
Extraction Buffer; Nitrate Reductase Enzyme; Griess Reagent A; Griess Reagent B; Standard
Sample Types
Leafy vegetables, root vegetables, crop plant homogenates, food products
Sample Volume
10 μL - 50 μL
Detection Principle
Quantification of vegetable nitrate is established through a specific substrate-mediated reaction. Signal intensity recorded via microplate reader or spectrophotometer is proportionally calibrated against analytical standard curves.
Detection Time
Within 1 hour
Detection Wavelength
540 nm
Required Equipment & Materials (Self-provided by User)
Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.
Storage
Store at -20°C.
Shipping Conditions
Shipped with ice packs.
Application Notes
Background
Vegetable nitrate is recognized as a fundamental component in biological systems. Its concentration levels are highly indicative of the functional state and metabolic vigor of the sample. In addition, assessing this component offers a reliable parameter for agricultural research or biological studies.
Application
Tailored for accurate quantification of vegetable nitrate across cell biology, organelle bioenergetics, and intracellular signaling cascades.
Key Features & Advantages
High Specificity; Stable Reaction; Broad Sample Compatibility
Operating Instructions
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.
For Research Use Only.
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