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The GlycoSens™ D-Lactic Acid (D-LA) Assay Kit utilizes a highly stable detection system tailored for accurate profiling of d-lactic acid. It combines streamlined sample handling with superior signal-to-noise ratios to support dependable biochemical assessment in research applications.
Product Type
Other Series
Target
D-LA
Kit Components
Reaction Buffer; Specific Substrate; Standard; Reaction Stop Solution
Sample Types
Tissues, other biological samples
Sample Volume
20 μL - 100 μL
Detection Principle
Target d-lactic acid (D-LA) in samples undergoes specific enzymatic conversion or chromatic reaction, resulting in a distinct optical absorbance change monitored spectrophotometrically. The variation in absorbance intensity directly correlates with the concentration of target D-LA.
Detection Time
Within 2 hours
Required Equipment & Materials (Self-provided by User)
Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.
Storage
Store at -20°C.
Shipping Conditions
Shipped with ice packs.
Target Information
Full Name
D-Lactic Acid
Alternate Names
D-Lactic Acid; D-Lactic Acid; D-LA
Application Notes
Background
In the context of biological research, d-lactic acid (D-LA) has emerged as a molecule of high interest. It participates directly in biosynthetic pathways, acting as a critical precursor for larger macromolecules. Notably, therefore, profiling its activity serves as a fundamental strategy for exploring complex biological phenomena.
Application
Ideal for investigating d-lactic acid (D-LA) activity and metabolic turnover during natural product research, growth factor signaling, and developmental morphogenesis studies.
Key Features & Advantages
Superior Accuracy; Low Background Noise; Highly Stable Signal Output
Operating Instructions
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.
2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.
3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.