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Quantitative Human dLK1 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX503

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Specifications
Product Description
The quantitative human dLK1 (glycosylated) sandwich ELISA kit is designed to detect human delta like 1 homolog (dLK1) levels. dLK1 is a transmembrane protein that functions as a ligand for Notch receptors. It is a key component of the Notch signaling pathway, which plays a critical role in cell fate determination and differentiation during development. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 0.255 ng/mL, which can accurately detect low concentrations of dLK1 in the sample.
Target
dLK1
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates, serum, plasma
Sample Volume
100 μL
Sensitivity
0.255 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
2 ng/mL-40 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Delta like 1 homolog
Alternate Names
dLK1; Delta like 1 homolog; FA1; PREF1; Protein delta homolog 1; Fetal antigen 1
Uniprot No.
N-Glycosylation Site
100, 165, 172
O-Glycosylation Site
94, 143, 163, 214, 222, 251, 256, 260
Application Notes
Application
The quantitative human dLK1 (glycosylated) sandwich ELISA kit is designed to quantitatively measure glycosylated dLK1 levels. This kit is a valuable tool for investigating the role of dLK1 and Notch signaling in various biological processes and diseases. Researchers can utilize it to measure dLK1 levels in different experimental settings. The kit has applications in developmental biology, stem cell research, and studies related to cancer and tissue regeneration.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-100%;
Plasma sample: n=4, 85-105%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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