Quantitative Human FPN (Glycosylated) Sandwich ELISA Kit

The quantitative human FPN (glycosylated) sandwich ELISA kit is designed to detect human ferroportin (FPN) levels. FPN is a transmembrane protein that is expressed in various cell types, including enterocytes and macrophages. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 0.125 ng/mL, which can accurately detect low concentrations of FPN in the sample.

FPN

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates, serum, plasma

100 μL

0.125 ng/mL

Quantitative sandwich ELISA

0.55 ng/mL-15 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Ferroportin

FPN; Ferroportin; SLC40A1; FPN1; Solute carrier family 40, member 1

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The quantitative human FPN (glycosylated) sandwich ELISA kit is used to measure FPN levels in biological samples. This kit is applicable in studies examining iron transport, iron deficiency, and iron overload disorders. It is also relevant to studies investigating the role of FPN in regulating systemic iron levels.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 80-95%;
Plasma sample: n=4, 85-100%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.