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Quantitative Human G6PC (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX387)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human G6PC (glycosylated) sandwich ELISA kit is designed to detect human glucose-6-phosphatase (G6PC) levels. G6PC is an enzyme, which is located in the endoplasmic reticulum. G6PC catalyzes the final step in gluconeogenesis. Gluconeogenesis is the production of glucose. G6PC plays a crucial role in regulating blood glucose levels. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.084 ng/mL, which can accurately detect low concentrations of G6PC in the sample.
Target G6PC
N-Glycosylation Site 96
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.084 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Glucose-6-phosphatase
Alternate Names G6PC; Glucose-6-phosphatase; G6PT
Uniprot No. P35575
Application The quantitative human G6PC (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated G6PC levels. It is useful for studying the role of G6PC in hepatic glucose production.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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