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Quantitative Human G6PC (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX387

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Specifications
Product Description
The quantitative human G6PC (glycosylated) sandwich ELISA kit is designed to detect human glucose-6-phosphatase (G6PC) levels. G6PC is an enzyme, which is located in the endoplasmic reticulum. G6PC catalyzes the final step in gluconeogenesis. Gluconeogenesis is the production of glucose. G6PC plays a crucial role in regulating blood glucose levels. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.084 ng/mL, which can accurately detect low concentrations of G6PC in the sample.
Target
G6PC
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.084 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Glucose-6-phosphatase
Alternate Names
G6PC; Glucose-6-phosphatase; G6PT
Uniprot No.
N-Glycosylation Site
96
Application Notes
Application
The quantitative human G6PC (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated G6PC levels. It is useful for studying the role of G6PC in hepatic glucose production.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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