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Quantitative Human ITGβ5 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX271

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Specifications
Product Description
The quantitative human ITGβ5 (glycosylated) sandwich ELISA kit is designed to detect human integrin β5 (ITGβ5) levels. ITGβ5 is a protein that is a component of integrin complexes. This protein plays a role in cell migration and angiogenesis. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.087 ng/mL, which can accurately detect low concentrations of ITGβ5 in the sample.
Target
ITGβ5
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.087 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Integrin β5
Alternate Names
ITGβ5; Integrin β5; ITG-B5
Uniprot No.
N-Glycosylation Site
347, 460, 477, 505, 552, 586, 654, 705
Application Notes
Application
The quantitative human ITGβ5 (glycosylated) sandwich ELISA kit provides a means for the quantitative analysis of glycosylated ITGβ5 levels in biological samples. Researchers use it in studies investigating cancer metastasis, angiogenesis, and wound healing. The kit is applicable in cell biology research.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-105%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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