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Quantitative Human PDGFRα (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX259

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Specifications
Product Description
The quantitative human PDGFRα (glycosylated) sandwich ELISA kit is designed to detect human platelet-derived growth factor receptor α (PDGFRα) levels. PDGFRα is a protein that functions as a receptor tyrosine kinase. This receptor is activated by platelet-derived growth factors, which play a role in cell proliferation, differentiation, and migration. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.099 ng/mL, which can accurately detect low concentrations of PDGFRα in the sample.
Target
PDGFRα
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.099 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Platelet derived growth factor receptor α
Alternate Names
PDGFRα; Platelet derived growth factor receptor α; CD140a; PDGFR2; CD140 antigen-like family member A
Uniprot No.
N-Glycosylation Site
42, 76, 103, 179, 353, 359, 458, 468
Application Notes
Application
The quantitative human PDGFRα (glycosylated) sandwich ELISA kit provides a means for the quantitative analysis of glycosylated PDGFRα levels in biological samples. Researchers use it in studies investigating cancer, particularly gliomas, and fibrosis. The kit is applicable in cancer research and the development of targeted therapies.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 90-105%;
Plasma sample: n=4, 95-110%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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