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Quantitative Human SCNN1γ (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX450

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Specifications
Product Description
The quantitative human SCNN1γ (glycosylated) sandwich ELISA kit is designed to detect human amiloride sensitive sodium channel subunit γ (SCNN1γ) levels. SCNN1γ is another subunit of the epithelial sodium channel (ENaC). Like SCNN1β, it contributes to the regulation of sodium reabsorption. ENaC plays a vital role in maintaining fluid and electrolyte balance. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.120 ng/mL, which can accurately detect low concentrations of SCNN1γ in the sample.
Target
SCNN1γ
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates
Sample Volume
100 μL
Sensitivity
0.120 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.55 ng/mL-15 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Amiloride sensitive sodium channel subunit γ
Alternate Names
SCNN1γ; Amiloride sensitive sodium channel subunit γ; ENaCg; SCNEG; Nonvoltage-gated sodium channel 1 subunit gamma
Uniprot No.
N-Glycosylation Site
209, 497
Application Notes
Application
The quantitative human SCNN1γ (glycosylated) sandwich ELISA kit is designed to measure glycosylated SCNN1γ levels in samples. This measurement is valuable in research related to sodium transport and kidney functions. The kit is applied in studies of conditions affecting electrolyte balance, which helps in understanding the role of SCNN1γ.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 90-105%;
Plasma sample: n=4, 80-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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