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Quantitative Human WNT9B (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX33

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Specifications
Product Description
The quantitative human WNT9B (glycosylated) sandwich ELISA kit is designed to detect human wingless type MMTV integration site family, member 9B (WNT9B) levels. WNT9B is a secreted glycoprotein that plays a role in various developmental processes, including kidney development, limb patterning, and tissue homeostasis, by activating Wnt signaling pathways. It is involved in cell fate determination and tissue morphogenesis. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.123 ng/mL, which can accurately detect low concentrations of WNT9B in the sample.
Target
WNT9B
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.123 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Wingless type MMTV integration site family, member 9B
Alternate Names
WNT9B; Wingless type MMTV integration site family, member 9B; WNT15
Uniprot No.
N-Glycosylation Site
99
Application Notes
Application
The quantitative human WNT9B (glycosylated) sandwich ELISA kit is valuable for researchers investigating kidney diseases, skeletal disorders, and cancer, where WNT9B signaling is implicated. By quantifying WNT9B, researchers can gain insights into its role in disease pathogenesis and explore potential therapeutic targets related to Wnt pathway modulation.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-100%;
Plasma sample: n=4, 85-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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