Recombinant Human Aggrecan (ACAN) protein (Pro34-Glu147) [His-tag and S-tag], E. coli

Recombinant Human Aggrecan (ACAN) protein (NP_001126.3) (Pro34-Glu147) was expressed in E. coli with a His-tag and S-tag at the N-terminus. The biological activity was determined by its binding ability in a functional ELISA. This product can be used in ELISA, WB, IP.

Determined by its binding ability in a functional ELISA.

Human

Pro34-Glu147

MHHHHHHSSGLVPRGSGMKETAAAKFERQHMDSPDLGTDDDDKAMADIGSEFPQPSPLRVLLGTSLTIPCYFIDPMHPVTTAPSTAPLAPRIKWSRVSKEKEVVLLVATEGRVRVNSAYQDKVSLPNYPAIPSDATLEVQSLRSNDSGVYRCEVMHGIEDSEATLE

His-tag and S-tag at the N-terminus

18.2 KDa

>95%, determined by SDS-PAGE and HPLC

<1 EU/μg, determined by the LAL method

Unconjugated

ACAN

Aggrecan

This gene is a member of the aggrecan/versican proteoglycan family. The encoded protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage. Mutations in this gene may be involved in skeletal dysplasia and spinal degeneration. Multiple alternatively spliced transcript variants that encode different protein isoforms have been observed in this gene.

AGC1; SEDK; AGCAN; CSPG1; MSK16; CSPGCP; SSOAOD; ACAN; Aggrecan