Recombinant Sonchus yellow net virus (SYNV) Spike glycoprotein (G) (18-632 aa), E. coli

Recombinant Sonchus yellow net virus (SYNV) Spike glycoprotein (NP_042285.1) (18-632 aa) was expressed in E. coli with a His-tag or Tag free. The biological activity was determined by its binding ability in a functional ELISA. This product can be used in ELISA, WB, and IP.

Determined by its binding ability in a functional ELISA.

Sonchus yellow net virus (SYNV)

18-632 aa

SWSLLGYQDDFSSKRSGALASNPTYNLPQDKGYGRDMYQPYYICEPDNDGSALTLPSWHY SCKESCMGNHLKRVVNITGARWNYVGISIPVFKIVTNEVCYTSHENVWGYCSQYQISRPV ATQKSDVSCITSSMWDNDKSPIGSLYNIVNSNEAECDYFSDITDCNRDYQIFKREGKLIK RSDDSPLELSIVTDGIRTDPASEYLSLDDVSWFWKLPNNDMSPPCGWEKTQKLSCSYTDT TDVIKCNSIGYTYNIQGISKKSTCAGNIYDTDGPFPFFYDAEEALMSTDDACGKAKQGKP DADIAFIEGVNRAFEDLELTYCSATCDLFARQGTPNEDHVLDTPIGTWRYVMRDNLDPAL VPCLPTSNWTISDPTTICHGKDHILVVDTATGHSGSWDTKKDYIITGEVCNTNNDEMGDD YDGMRDKILRGETIEIKFWTGDIIRMAPPYDNPEWIKGSVLFRQNPGWFSSVELNKDMIH TRDNITDLLTVMVQNATAEVMYKRLDPKTMKHILFAEIVDGVGNVSGKISGFLTGLFGGF TKAVIIVASLAICYIVLSVLWKVRLVASIFNSAKKKRVRISDILDEEPHRIQQSRPTLSR KKKTRESIQMLLNDI

His-tag or Tag free

>90%, determined by SDS-PAGE.

Unconjugated

G

Spike glycoprotein

This protein forms spikes on the surface of the virion. It is responsible both for the binding of the virus to susceptible host cells and for inducing the uptake of the virus by the cell. The interaction between the internal components of the virion and the portion of the glycoprotein exposed on the cytoplasmic face of the plasma membrane probably directs envelopment and virus budding.

Spike glycoprotein