GlycoErase™ SIGLEC12 Knockout A549 Cell Line (CAT#: GLJF-0825-JF856)

Description	GlycoErase™ SIGLEC12 knockout A549 cell line is a human lung epithelial cancer model with SIGLEC12 expression removed. SIGLEC12 belongs to the SIGLEC3-like subfamily, defined by an extracellular V-set domain followed by two C2-set domains and cytoplasmic tyrosine-based motifs, including ITIM and SLAM-like sequences. Upon phosphorylation, SIGLEC12 can recruit SHP1 and SHP2 phosphatases, functioning as an inhibitory receptor that negatively regulates macrophage signaling. The knockout of SIGLEC12 in the A549 background provides a relevant system for dissecting immune inhibition, macrophage-mediated pathways, and receptor-phosphatase signaling, while supporting applications in immunology, signal transduction research, and therapeutic development.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:3~1:6
Cell Line	A549
Primary Disease	Non-small cell lung cancer
Lineage	Lung
Lineage Subtype	Non-small cell lung cancer

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	F-12K & FBS & Penicillin/Streptomycin
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	SIGLEC12
Full Name	Sialic Acid Binding Ig Like Lectin 12
Alternative Name	S2V, SLG, SIGLECL1, Siglec-XII, Sialic Acid Binding Ig Like Lectin
Location	19q13.41
Gene ID	89858
Summary	Sialic acid-binding immunoglobulin-like lectins (SIGLECs) are a family of cell surface proteins belonging to the immunoglobulin superfamily. They mediate protein-carbohydrate interactions by selectively binding to different sialic acid moieties present on glycolipids and glycoproteins. This gene encodes a member of the SIGLEC3-like subfamily of SIGLECs. Members of this subfamily are characterized by an extracellular V-set immunoglobulin-like domain followed by two C2-set immunoglobulin-like domains, and the cytoplasmic tyrosine-based motifs ITIM and SLAM-like. The encoded protein, upon tyrosine phosphorylation, has been shown to recruit the Src homology 2 domain-containing protein-tyrosine phosphatases SHP1 and SHP2. It has been suggested that the protein is involved in the negative regulation of macrophage signaling by functioning as an inhibitory receptor. This gene is located in a cluster with other SIGLEC3-like genes on 19q13.4. Alternative splicing results in multiple transcript variants.