GlycoErase™ SIGLEC15 Knockout CHO-K1 Cell Line (CAT#: GLJF-0825-JF869)

Description	GlycoErase™ SIGLEC15 knockout CHO-K1 cell line is a CHO-K1 model lacking SIGLEC15 expression. SIGLEC15 regulates actin cytoskeleton organization, bone resorption, and osteoclast development, and is associated with plasma membrane complexes. Removal of SIGLEC15 in CHO-K1 provides a system to explore osteoclast biology, skeletal disease mechanisms, and cytoskeletal regulation, with applications in bone metabolism, therapeutic validation, and immune modulation.
Product Type	KO Cell Lines
Species	Hamster
Cell Morphology	Epithelial, adherent
Passage Ratio	1:3~1:5
Cell Line	CHO-K1
Lineage	Chinese hamster ovary

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	FBS & Penicillin/Streptomycin & Proline & RPMI
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	SIGLEC15
Full Name	Sialic Acid Binding Ig Like Lectin 15
Alternative Name	CD33L3, HsT1361, SIGLEC-15, Sialic Acid Binding Ig Like Lectin
Location	18q21.1
Gene ID	284266
Summary	This protein is predicted to regulate actin cytoskeleton organization, bone resorption, and osteoclast development. It is expected to be located in the membrane and to be part of a protein-containing complex, functioning actively in the plasma membrane.