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GlycoFlux™ AAV1-Human B3GNT2 Viral Particle

CAT#: GLJF-1025-JF784

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Specifications
Description
GlycoFlux™ AAV1-B3GNT2 Viral Particle ensures durable B3GNT2 expression for elongation of poly-N-acetyllactosamine chains with preference for lacto-N-neotetraose, and this product demonstrates consistent transduction in liver and heart, enabling long-term modulation of glycan backbone architecture in vivo.
Product Type
Recombinant AAV1
Target
B3GNT2
Species
Human
Promotor
CMV, CAG, TRE, or other promoters
Tissue Tropism
Smooth muscle, Central Nervous System (CNS), Retina, Pancreas, Inner Ear, Heart, Liver
Packaging System
AAV Triple-Plasmid Transfection System
Packaging Cell
HEK293T
Application
This AAV1 viral particle is primarily used for overexpressing target genes in muscle tissues (smooth muscle, heart) and the central nervous system (CNS) / sensory organs (retina, inner ear). It is an ideal tool for research and pre-clinical studies focused on myopathies, cardiac disorders, and certain neurological or sensory genetic diseases.
Shipping
Shipped on dry ice, with temperature maintained at ≤ -80°C.
Storage
Store at -80°C to preserve viral titer and stability.
Handling Notes
Store this product at -80°C and minimize freeze-thaw cycles to preserve viral titer. For safety, handle in a certified biological safety cabinet and wear appropriate PPE (lab coat, gloves, and eye protection).
Product Disclaimer
This product is intended for research use only and is not approved for diagnostic procedures or clinical applications. Users are solely responsible for implementing appropriate safety practices and complying with all applicable laws, regulations, and institutional policies. While Creative Biolabs is committed to rigorous quality control, no guarantee is made regarding performance in any particular assay or application.
Quality Control
Titer Assay
qPCR/TCID50
Mycoplasma Test
No mycoplasma contamination was detected.
Sterility
Sterility testing verified the absence of microbial contamination.
Transduction Evaluation
Upon request, Creative Biolabs performs in vitro or in vivo transduction assays to assess AAV delivery to target cells and to quantify transgene expression and function.
Insert Identity Confirmation
All AAV preparations from Creative Biolabs are verified for vector-genome identity by PCR. In this assay, target cells are transduced with serial dilutions of the AAV preparation, harvested after a short incubation, and genomic DNA is isolated; a defined region of the packaged transgene is then PCR-amplified to confirm the expected sequence.
Target Information
Full Name
UDP-GlcNAc:BetaGal Beta-1,3-O-acetylglucosaminyltransferase 2
Alternative Name
B3GNT1; BETA3GNT; B3GN-T1; B3GNT-2; B3GN-T2
Location
2p15
Gene ID
10678
Summary
Type II transmembrane protein that prefers lacto-N-neotetraose as a substrate and participates in the biosynthesis of poly-N-acetyllactosamine chains.
For Research Use Only.
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