GlycoSens™ Acetyl-CoA Assay Kit, Spectrophotometry

The GlycoSens™ Acetyl-CoA Assay Kit features an optimized micro-volume detection system designed for sensitive measurement of acetyl-CoA levels across diverse research samples. The assay components are ready-to-use, ensuring high analytical reproducibility with minimal sample requirements.

Tricarboxylic Acid Cycle Series

Acetyl-CoA

Extraction Buffer; Reaction Reagent A; Reaction Reagent B; Standard

Tissues, other biological samples

10 μL - 50 μL

Quantification of acetyl-CoA is established through a specific substrate-mediated reaction. Signal intensity recorded via microplate reader or spectrophotometer is proportionally calibrated against analytical standard curves.

Within 2 hours

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Acetyl-CoA

Acetyl-CoA

Research highlights acetyl-CoA as a significant participant in key metabolic and regulatory cascades. Its concentration levels are highly indicative of the functional state and metabolic vigor of the sample. Assessing this component offers a reliable parameter for agricultural research or biological studies.

Tailored for accurate quantification of acetyl-CoA across cell biology, organelle bioenergetics, and intracellular signaling cascades.

Rapid Reaction Protocol; Stringent Quality Control; Enhanced Sensitivity

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.