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The GlycoSens™ ADPG Pyrophosphorylase Assay Kit offers an advanced quantitative methodology for evaluating ADPG pyrophosphorylase concentration with high specificity. Designed to minimize matrix background interference, this kit facilitates high-throughput scientific profiling and consistent experimental turnaround.
Product Type
Carbohydrate Metabolism Series
Target
ADPG Pyrophosphorylase
Kit Components
Reaction Buffer; Specific Substrate; Standard; Reaction Stop Solution
Sample Types
Tissues, other biological samples
Sample Volume
10 μL - 50 μL
Detection Principle
The system measures chromatic product formation derived from target ADPG pyrophosphorylase activity. Absorbance changes tracked at optimal wavelengths allow precise quantitative determination of ADPG pyrophosphorylase levels.
Detection Time
Within 1 hour
Required Equipment & Materials (Self-provided by User)
Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.
ADPG pyrophosphorylase is an integral element required for robust cellular function and metabolism. This catalytic protein strictly regulates substrate turnover to meet the dynamic physiological needs of the cell. Moreover, thus, accurate determination using this assay supports advanced exploration in biochemistry and molecular biology.
Application
Tailored for accurate quantification of ADPG pyrophosphorylase across cell biology, organelle bioenergetics, and intracellular signaling cascades.
Key Features & Advantages
Superior Accuracy; Low Background Noise; Highly Stable Signal Output
Operating Instructions
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.
2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.
3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.