GlycoSens™ β-Hydroxybutyrate Assay Kit, WST Method

The GlycoSens™ β-Hydroxybutyrate Assay Kit utilizes a highly stable detection system tailored for accurate profiling of β-hydroxybutyrate. It combines streamlined sample handling with superior signal-to-noise ratios to support dependable biochemical assessment in research applications.

Basal Metabolic Series

β-Hydroxybutyrate

Extraction and assay buffer; Substrate Mix A; Substrate Mix B; Reaction developer

Tissues, other biological samples

10 μL - 50 μL

Target β-hydroxybutyrate in samples undergoes specific enzymatic conversion or chromatic reaction, resulting in a distinct optical absorbance change monitored spectrophotometrically. The variation in absorbance intensity directly correlates with the concentration of target β-hydroxybutyrate.

30-60 minutes

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

β-Hydroxybutyrate

β-Hydroxybutyrate

Known for its specific biological characteristics, β-hydroxybutyrate provides deep insights into cellular dynamics. It actively circulates within the biological matrix, acting as a crucial building block or signaling molecule. Investigating its functional state is crucial for identifying novel biomarkers and therapeutic targets.

Optimized for high-throughput profiling of β-hydroxybutyrate in functional metabolomics, secondary metabolite profiling, and environmental adaptation research.

Rapid Reaction Protocol; Stringent Quality Control; Enhanced Sensitivity

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.